Journal: International Journal of Molecular Sciences

Article Title: Effects of Palm Oil Tocotrienol-Rich Fraction (TRF) and Carotenes in Ovalbumin (OVA)-Challenged Asthmatic Brown Norway Rats

doi: 10.3390/ijms20071764

Figure Lengend Snippet: Effects of palm oil TRF, carotenes, and dexamethasone on pro-inflammatory cytokines, ( a ) IL-1β, ( b ) IL-6, and ( c ) TNF-α, in the plasma of Brown Norway rats challenged with OVA. Data are presented as the means ± SD, n = 10 rats per group. a P < 0.05 versus the control group. b P < 0.05 versus the OVA-challenged group.

Article Snippet: Standard enzyme linked immunosorbent assay (ELISA) kits used for the determination of rat IL-β, IL-6, IL-4, IL-13, TNF-α (Novateinbio, Woburn, Massachusetts, USA), CRP (eBioscience, Affymetrix, Santa Clara, California, USA), IgE, LTB-4, and LTB-5 (MyBioSource, San Diego, California, USA) were utilized.

Techniques:

Journal: Evidence-based Complementary and Alternative Medicine : eCAM

Article Title: Dracorhodin Perchlorate Accelerates Cutaneous Wound Healing in Wistar Rats

doi: 10.1155/2017/8950516

Figure Lengend Snippet: Effect of DP ointment on the cytokine production in rat skin wound tissue. Wound biopsies were obtained from the rats treated with DP ointment and control ointment on days 0, 3, 7, and 14. (a) TNF- α and (b) IL-1 α were assayed through ELISA. Data are expressed as mean ± SEM, ∗ p < 0.05 compared with the vehicle control group.

Article Snippet: The following chemicals and kits were used: DP (≥99%; Pharmaceutical Inspection Institute, Harbin, China), VEGF and EGF antibodies (Sangon Biotech Institute, Shanghai, China), and TNF- α and IL- β kits (Nanjing Jiancheng Bioengineering Institute, Nanjing, China).

Techniques: Ointment, Control, Enzyme-linked Immunosorbent Assay

Journal: Frontiers in Veterinary Science

Article Title: Targeting Soluble Epoxide Hydrolase and Cyclooxygenases Enhance Joint Pain Control, Stimulate Collagen Synthesis, and Protect Chondrocytes From Cytokine-Induced Apoptosis

doi: 10.3389/fvets.2021.685824

Figure Lengend Snippet: Cytokine response associated with synovitis during inhibition of soluble epoxide hydrolase (sEH) and cyclooxygenases (COX). Forty-eight-hour time course (top row) and aggregate area under curve (AUC, bottom row) for synovial fluid concentrations of interleukin (IL)-1β (A,D) , IL-6 (B,E) , and tumor necrosis factor (TNF)-α (C,F) in an equine synovitis model induced by intra-articular injection of lipopolysaccharide (LPS; 3 μg) into the radiocarpal joint ( n = 6 horses) as measured using ELISA techniques. Horses were treated intravenously with the sEH inhibitor trans -4-{4-[3-(4-Trifluoro-methoxy-phenyl)-ureido]-cyclohexyloxy}-benzoic acid (TUCB), the non-selective COX inhibitor phenylbutazone (PBZ) or both, at the indicated doses marked above (top row) or below (bottom row) the graphs, at the same time as the intra-articular LPS injection. Top row: Data are shown as mean ± SEM. Bottom row: Data are shown as individual values (symbols) and mean ± SEM (columns), and p -values are shown above each respective comparison (Mixed-effects model corrected for multiple comparisons by controlling the False Discovery Rate).

Article Snippet: To determine the effect of COX and sEH inhibition on articular cartilage matrix, prostanoid production, and pro-inflammatory biomarkers during synovitis, we assayed (ELISA) all synovial fluid samples for type II collagen synthesis (CPII) and degradation (C2C) biomarkers (IBEX Technologies, Quebec, CAN), prostaglandin (PG) E 2 , thromboxane (Tx) B 2 (Enzo Life Sciences, Farmingdale, NY, USA), interleukin (IL)-β, IL-6, and tumor necrosis factor (TNF)-α (equine specific assays from Genorise Scientific, Inc., Glen Mills, PA, USA).

Techniques: Inhibition, Injection, Enzyme-linked Immunosorbent Assay, Comparison